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Distortion was also noticed in samples made up of lipids, particularly in the Hoki roe homogenate. Pokhariyal et al. [49] noted that the complexity of protein bands from the SDS-Website page was greater during the presence of lipid, which resulted in distortion of protein bands.
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The data received from your time class hydrolysis of Hoki and Gemfish roe homogenates (Figure one) exhibit progressive hydrolysis by the 3 proteases used in the examine as demonstrated by the increase in L-serine equal contents over the hydrolysis time. For Hoki roe, Alcalase exhibited an In general larger DH in comparison with HT and FP-II, and also the hydrolysis with FP-II was lessen than that of HT. These final results are in agreement With all the findings of Ahmmed et al. [13] who reported Alcalase to possess a much better hydrolysis capacity with Hoki roe homogenate than HT and FP-II, underneath the exact same hydrolysis problems, and the DH is improved with rising the protease focus.
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